SOP FOR OPERATION AND CALIBRATION OF HEATING BLOCK

1.0          Objective:

               The objective of this Standard Operating Procedure is to lay down the procedure for operating and calibration of Heating Block.

2.0          Scope:

               This SOP covers operating and calibration of Heating Block

3.0          Responsibility:

               Microbiologist: Responsible for operation, calibration and maintenance of the instrument. Maintain the required record.

               QA Officer/QAM: Review the records and governing the document.

4.0          Procedure:

4.1          Ensure that the instrument is visibly clean and free from dust.

4.2      Switch ‘ON’ the mains switch. Switch on the power switch of the instrument and observe that the LED of Temperature indicator displays the ambient value.

4.4          Press ‘SET TEMP.’ key (Red Button) to pre-set the desired performance parameter.

4.5        The LED shall display the lat setting. Turn the setting knob clockwise for decrement until the LED displays the desired set value. Use the second knob for fine setting if required. Depress (Release) the SET key.

4.6          The LED shall regain to display the ambient value of the chamber.

4.7          Now the unit will automatically perform to attain the set temperature with the on and off of the heater.

4.8         The heater selection (heater capacity) switch is provided having positioned 37°, 45°and 75° or High.

4.9        Select the switch initially at HIGH to attain the set point quickly. Turn it to LOW soon the display is reaching near to the set point for fine and accurate control, Thus to prevent the shooting up the temperature from the set value.

4.10      Select the LOW position if the set point is nearing the ambient for accurate and fine performance.

5.0          Calibration:

5.1          Operate the instrument as per above procedure.

5.2      Set the temperature knob at desire temperature at which temperature you want to calibrate.

5.3          Place a calibrated thermometer of appropriate range in the chamber of the heating block.

5.4          Set the three different temperature and note down the constantly result for a period of 60   minutes at interval of 15 minutes.

5.5          Note down the temperature in the calibration format .

5.6                    Acceptance Criteria: ↑± 1.0 ° C for Set Temperature

6.0                    Routine maintenance:

6.1          Clean the instrument with dry cloth.