SOP FOR METHOD OF PREPARATION & STANDARDIZATION OF VOLUMETRIC SOLUTION

0.02M SODIUM HYDROXIDE

2.4% W/V GLACIAL ACETIC ACID SOLUTION

Method of Preparation:

Dilute 2.4 ml of glacial acetic acid in water to produce 100 ml.
Weight of glacial acetic acid:

[W₁ :______________ mg, W₂:______________mg, W₁- W₂: _______________mg ]

Where, ever in the protocol

W₁           = Weight of sample,

W₂         = Weight of paper after transferring sample,

W₁- W₂   = Actual Weight of sample.

Name of Chemical	Batch No.	Make
Glacial acetic acid

Storage :
Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper

AMMONIUM STANDARD SOLUTION [1 ppm NH4]

METHOD OF PREPARATION:

Dilute 10 ml of 0.0741% w/v solution of Ammonium Chloride to 25.0 ml with ammonia free water. Dilute 1 volume of this solution to 100 volumes with ammonia free water immediately before use.

[Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.

0.1 % W/V HYDROCHLORIC ACID

Method of Preparation

Dilute 1 ml of hydrochloric acid with water to produce 100 ml.

[Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.]      




Sensitivity :
Storage :

Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper

Ammonia (10 M)

METHOD OF PREPARATION: 

Dilute 75 ml of strong ammonia solution to 100 ml with distilled water.

                                                  

[Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.]                               

ACETATE BUFFER pH 3.5

METHOD OF PREPARATION:

Dissolve 25 g of ammonium acetate in 25 ml of water and add 38 ml of 7M hydrochloric acid. Adjust the pH to 3.5 with either 2M hydrochloric acid or 6M ammonia and dilute to 100 ml with distilled water.

 [Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.]   

AMMONIA SOLUTION 6M

METHOD OF PREPARATION: 

Dilute 45 ml of strong ammonia solution to 100 ml with distilled water.

 [Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.]       

AMMONIUM CHLORIDE SOLUTION

METHOD OF PREPARATION: 

Dissolve 0.0741 gm of Ammonium Chloride in distilled water to produce 100 ml.

[Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.] 

AMMONIA AMMONIUM CHLORIDE SOLUTION

METHOD OF PREPARATION:

Dissolve 67.5 gm of Ammonium Chloride in 740 ml of strong Ammonia solution (25 %) and add sufficient distilled water to produce 1000 ml.

[Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.]                               

CHLORIDE STANDARD SOLUTION [5 PPM Cl]

METHOD OF PREPARATION:

Dilute 1 ml of 0.0824% w/v solution of Sodium Chloride to 100.0 ml with distilled water.

[Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.]              

DISODIUM EDETATE 0.01 M

METHOD OF PREPARATION:

Dilute 10 ml of 0.1 M EDTA to 100 ml with distilled water.

 [Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.]    

DIPHENYLAMINE SOLUTION

METHOD OF PREPARATION:

Dissolve 0.1 g of Diphenylamine in 100 ml of Sulphuric acid.

 [Store this solution at room temperature in Borosil amber glass bottles with well-fitted suitable stopper.]     

POTASSIUM CHLORIDE SOLUTION 3 M

METHOD OF PREPARATION: 

Dissolve 22.3 gms of Potassium chloride in 100 ml of water. 

[Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.] 

HYDRAZINE MOLYBDATE REAGENT

METHOD OF PREPARATION: 

Dissolve 0.1 gm ammonium Molybdate in 10 ml water containing 1.5 ml of Sulphuric acid. Dilute to about 90 ml with water & add 1 ml of 0.15 % w/v solution of Hydrazine Sulphate & sufficient water to produce 100 ml.

[Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.]

HYDROCHLORIC ACID 2.0 M

METHOD OF PREPARATION: 

Dilute 17 ml of Hydrochloric Acid with sufficient water to produce 100 ml.

 [Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.] 

HYDROCHLORIC ACID 3.0 M

METHOD OF PREPARATION: 

Dilute 63.8 ml of Hydrochloric Acid with sufficient water to produce 250 ml.

 [Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.]   

HYDROCHLORIC ACID 7 M

METHOD OF PREPARATION: 

Dilute 59.5 ml of Hydrochloric Acid with distilled water to produce 100 ml.

 [Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.]    

IODINE SOLUTION 0.1 M

METHOD OF PREPARATION: 

Dissolve 40 gms of Potassium iodide in the minimum amount of water, add 26 gms of Iodine, allow to dissolve and add sufficient water to produce 1000 ml.

[Store this solution at room temperature in Borosil amber glass bottles with well-fitted suitable stopper.]  

LEAD STANDARD SOLUTION [0.1 % pb]

METHOD OF PREPARATION:

Dissolve 0.4 gm of lead [II] nitrate in sufficient water containing 2 ml of nitric acid to produce 250 ml.

[Store this solution at room temperature in Borosil glass bottles with well-fitted suitable stopper.]