SOP FOR OPERATION OF CENTRIFIGUE MACHINE

1.0          Objective:

               The objective of this Standard Operating Procedure is to lay down the procedure for operating the Centrifuge.

2.0          Scope:

               This SOP covers operating procedure of Centrifuge .

3.0          Responsibility:

               Junior Research officer/ Research Officer/ Microbiologist: Responsible for operation of the instrument as per procedure.

              QA Officer/QA Manager: Review the records and governing the document.

4.0          Procedure:

4.1                       Open the lid of the instrument. Fill the material in tubes. The volume of each tube should be equal. Load the tubes in instrument’s test tube stand. If all tubes are not filled up with material, please put the filled test tubes on opposite sides, so as to maintain the balance.

4.2                       Connect the three-pin plug to the mains. Switch the mains ON.

4.3                       Switch the instrument ON.

4.4                     Regulate the speed control knob from minimum to maximum gradually till desired speed is reached.

4.5                       The desired time duration for the test can be adjusted with the help of timer knob.

4.6                       Now wait till the operation of centrifuge is over.

4.7                       When the timer will reach to ’00’, the function will stop automatically.

4.8                       Allow the centrifuge to reach at stop position and read the speed meter indicating “00”.

4.9                       Open the lid, remove the test tubes and observe the result.

4.10                   After completion of work switch ‘OFF’ the instrument and the mains.