SOP FOR OPERATION AND CALIBRATION OF ANTIBIOTIC ZONE READER

1.0          Objective:

               To provide written procedure for operation and calibration of Antibiotic Zone Reader.

2.0          Scope:

               This SOP covers the operation and calibration of Antibiotic Zone Reader.

3.0          Responsibility:

               Microbiologist: Responsible for operation, calibration and maintenance of the instrument. Maintain the required record.

               QA Officer/QAM: Review the records and governing the document.

4.0          Procedure:

4.1          Ensure that the instrument is visibly clean and free from dust.

4.2          Switch ‘ON’ the mains switch.

4.3         Turn the left side drum to its maximum clockwise position, so that Zero Marking on the scale of the right side drum coincides with the Zero marking on the Vernier scale.

4.4         Keep the Petri-dish on the Aluminum plate and rotate it manually; so that one end of the Zone image touches the Mark Line on the Prism.

4.5       Now turn the left-side drum slowly, in anti-clockwise position, till the other end of the Zone image touches the Mark Line on the Prism.

4.6         Note the reading directly on the Right side drum scale. The scale is marked from 0 to 35 mm with each division of 0.2 mm and the Vernier scale is marked with each division of 0.02 mm.

5.0          Calibration:

5.1          Operate the instrument as per above procedure.

5.2          Put the standard coin in the appropriate place on antibiotic zone reader.

5.3          Measure the zone of standard coin and compare with the standard coin result and observed result.

5.4         Record the result in the calibration format 

6.0                    Routine maintenance:

6.1                    Clean the instrument with dry cloth.

6.2                    Clean glass & lance with dry cloth or tissue paper.