SOP For PREPARATION OF MICROBIOLOGICAL MEDIA

1.0          Objective:

               To provide written procedure for the Preparation of Microbiological Media.

2.0          Scope:

               This SOP covers preparation of all media used in microbiological analysis 

3.0          Responsibility:

               Microbiologist: Responsible for preparation, handling the media and maintain the necessary record.

              QA Officer/QA Manager: Review the records and governing the document.

4.0          Procedure:

4.1          Check the expiry of the media before using the same.

4.2          Weigh the media as per the directions given on the respective label of media                          bottles / containers. Dilute with purified water as directed on the label. Check the pH of     the media if required.

4.3          Warm, if necessary closes the flask or the container with non-absorbant cotton plug            tightly. Cover the non-absorbant cotton plug with aluminium foil.

4.4          If necessary, dispense the media in flasks or test tubes as required and close it with non-          absorbant cotton plug and aluminium foil.

4.5          Sterilize the media by autoclaving at 121° C for 15 minutes or as directed on the label.

4.6          Record the autoclave load No. and other details in autoclave usage log book 

4.7          Record the details of media consumption in media consumption record book 

4.8          Check and conform the pH of media after sterilization using pH strip.

5.0          Precautions:

5.1          Do not over autoclave the media.

5.2          Do not re-autoclave the media.

5.3          Ensure the dissolution of dehydrated media before autoclaving.

5.4          Do not use the dehydrated media that shows discoloration, caking or any sign of deterioration.