SOP for CLEANING PROCEDURE OF MICROBIOLOGICAL LABORATORY

1.0          Objective:

               This SOP describes the procedure to be followed for Cleaning of Microbiology                      Laboratory.

2.0          Scope:

               This SOP covers the Cleaning of Microbiology Laboratory 

3.0          Responsibility:

          Laboratory Assistant: Responsible for the cleaning of the laboratory as per procedure.                 

               Microbiologist: Responsible for to check the cleaning of microbiology department.

              QA Officer/QA Manager: Governing the document.

4.0          Procedure:

4.1          Clean the floor with 1 % Teepol by wet mopping.

4.2          Clean the floor by wet mopping using the following disintefectants :

4.2.1       Dettol solution         :  5 % v/v (Dilute 50 ml to 1 litre with purified water).

4.2.2       Savlon solution        :  5 % v/v (Dilute 50 ml to 1 litre with purified water).

4.2.3       Triple 256 solution   :  1 ml to 256 ml with purified water.

4.3          Prepare fresh solutions every time.

               Monday, Thursday       :  Dettol solution

               Tuesday, Friday           :  Savlon solution

               Wednesday, Saturday  :  Triple 256 solution

4.4          Clean the Laminar air flow bench by 70 % v/v Isopropyl alcohol.

4.5          Clean the sides and top of the Laminar air flow with wet mop of Dettol / Savlon or          Triple 256 solution.      

4.6          Clean the incubator with 5 % v/v Dettol solution or 70 % v/v Isopropyl alcohol.

4.7              Cleaning schedule shall be as follows :

Sr. No.	Area	Cleaning Agent	Frequency
1	Laminar Air flow work bench	Mopping with 70 % v/v IPA	Before and after each work
2	Out side wall and Top of Laminar Air flow	5 % v/v Dettol solution, 5 % v/v Savlon solution or Triple 256 solution	Once Daily
3	Floor	5 % v/v Dettol solution, 5 % v/v Savlon solution or Triple 256 solution	Twice Daily
4	Window, Door, Glass-panes	Wet mop with 70 % v/v IPA	Weekly Once
5	Walls and ceiling	Cleaning with 70 % v/v IPA	Fortnightly
6	Light Fixtures	Dry Cloths	Fortnightly

5.0          Precautions

5.1          Wear nose mask while entering the Microbiology sterile area.

5.2          Switch off the tube light or any other electrical fixtures while cleaning them. Do not touch any eclectically equipment with wet hand.

5.3                       Use either soft plastic broom or clean cotton mops depending on the requirements.

5.4          Cleaning items for microbiology area should not be used for other area.

5.5          Avoid walking on wet floor after swabbing.

5.6          Change the disinfectant solution, used for swabbing of the floors as soon as it becomes dirty.