SOP FOR OPERATING PROCEDURE OF GAS CHROMATOGRAPHY AGILENT 6890N

1.0                    Objective:

               The objective of this Operating procedure is to lay down the procedure for Operating the Gas Chromatography Agilent 6890N.

2.0                      Scope:

               This procedure covers operation of Gas Chromatography.

3.0                    Responsibility:

              Jr. Research Officer, Research Officer: Responsible for operation and maintenance of

               the instrument as per procedure.

Head of Department: Responsible for maintenance, timely as per   schedule.

QA Officer/QA Manager: Review the records and governing the document

4.0          Procedure:

4.1       

   I.     Ensure that the instrument is visibly clean and free from dust.

  II.     The room temperature should be maintained between 10 to 30°C and relative humidity 

            Should be maintained between 30 to 80 %

III.    Avoid direct exposure to air-blow from air conditioners.

IV.    Avoid exposure to direct sunlight and vibrations.

V.     Avoid from equipment which generates strong magnetic field.

4.2          Start UP Procedure:

 4.2.1       Affix the require column (Capillary or packed) in to the column oven.

 4.2.2       Open the valve of gas cylinder of Z - air, Hydrogen and carrier gas (Nitrogen or Helium) and checked the pressure and leakage with 50 % v/v Isopropyl Alcohol solution.      

 4.2.3       Now Switch ON the Mains and Instrument. Switch on the computer.

4.3          Setting Instrument Parameters:

4.3.1      After Switch On instrument will display Oven Off message. Press oven key and press on key. The instrument started temperature increase of previous set temperature value.

4.3.2     Press Oven key, Add initial temperature, initial time and Rate (if required) with numerical key and press Enter key to confirm the vale.

4.3.3     If adding rate 1 the next oven program display, feed the required temperature, time and rate and then press Enter key to confirm the value. If column (Oven) temperature is   Isothermal condition the rate is off.  

4.3.4      Set the Oven Final Temperature below 20° C of Maximum Column temperature or as per procedure requirement.

4.3.5      Press ‘Back inlet’ key select mode split or split-less, add require inlet temperature with numerical key and press ‘Enter’ key to confirm the value.

4.3.6         Press ‘Front Det.’ key, add require detector temperature with numerical key and press ‘Enter’ key to confirm the value. Now increase the temperature, if temperature comes out at 150° C message display ‘FLAME OUT’.

4.3.7             Press ‘Front Det.’ Key and select Flame with ▲ and ▼ key select flame on with press ‘On’ key. Igniting message display and ignite the flame with gas lighter on detector side. The display shows the flame indicator in a numerical value.

4.3.8      Press ‘Status’ key and check the fed parameters again with ▲ and ▼ key to confirm the value. Now instrument is ready, the message will display “Instrument ready for Injection’.

4.3.9           Press “Signal 1” key select Zero with▲ and ▼ key, press ‘On’ key to flame numerical value should be zero and base line detection started to zero mv.

4.3.10  Now inject the solution with syringe. If used manual operation press ‘START’ key immediately after inject the solution.

4.4                     Software Operation:

4.4.1    Double click on the instrument1 (online) & instrument1 (offline) icon from the desktop;

             create new method from instrument1 (offline) and load method in instrument1 (online)

 from file menu. Apply the chromatographic condition and enter ok. And view the run

 signal window, After completion of the run, and open the instrument offline select the file 

 and] integrate the peak using integration menu, go to calibration task icon and enter the

             component name and click on ok, then print the chromatogram by clicking print option

             from file menu.

4.4.2  Condition the column at 280°C for 10 min. Enter the programming condition of the                        sample through micro syringe, when G.C shows ready signal.

4.4.3  Cool the G.C after completion of the analysis & switch it off. Shut down the computer, 

             mains power supply. Close the gas supply from the distribution panel & from cylinder.

5.0       Routine Maintenance:

5.1       Clean the all out side part of instrument.

5.2       Clean the injector and injector liner. Change the glass wool of injector liner. Change the

 septa of injector port if  required.

5.3         Check the all connection of gases for leakage.