SOP FOR DISPOSAL OF USED AND WASTE MICROBIOLOGICAL MEDIA

1.0          Objective:

               The purpose of this SOP is to provide guidelines for the Disposal of used & waste media in  microbiology laboratory.

2.0          Scope:

               This SOP covers  disposal of used and waste media from microbiology laboratory.

3.0          Responsibility:

            Microbiologist: Responsible for dispose the media as per guideline and maintain the necessary record.

              QA Officer/QA Manager: Review the records and governing the document.

4.0          Procedure:

4.1          Disinfect the glassware containing used media and other accessories used for                     microbiological testing as follows :

4.1.1       Used media: Pour 5 to 10 ml of 2 % v/v Phenol or 5 % v/v Dettol solutions to each of the glassware containing used media for disposal. Use the solutions alternately.

4.1.2       Allow to stand for 30 minutes.

4.2          Sterilize the glassware by autoclaving 15 lbs (at 121° C) for 30 minutes.

4.3       After the pressure in the autoclave is released, unload the glassware in hot condition                 and drain the liquid media in the washing area.

4.4          Wash the glassware .

4.5          After washing the destruction load, clean the washing area with 5 % v/v Dettol.

4.6          Wash the autoclave with 5 % v/v Dettol solution and then with tap water thoroughly before using the autoclave again.

4.7          Enter the details in disposal of microbiological media log book

4.8       CULTURE TUBES: Disinfect media tubes containing cultures by the above procedure            after the freshly sub-cultured is done and growth observed.

4.9    PIPETTES: Dip used pipettes in a cylinder or waste bin containing disinfectant. Allow                        to stand for 30 minutes and wash the pipettes.