SOP FOR PURITY OF THE CULTURES AFTER SUB-CULTURING

1.0              Objective:

            To provide written procedure for Confirmation of the purity of the cultures after sub culturing.

2.0              Scope:

            This SOP covers all microbial cultures used in the microbiological analysis 

3.0              Responsibility:

            Microbiologist: Responsible for preparation of media, handling the organism and maintain the necessary record.

            QA Officer/QA Manager: Review the records and governing the document.

4.0              Procedure:

4.1       Observe the growth characteristics after sub culturing,

4.2       If satisfactory growth observed, then proceed for the confirmation of the purity of the    

            cultures prior to its use in testing.

4.3              Pure cultures are obtained by further sub-culture onto plates of selective media as required.

4.4              Each pure culture is finally confirmed by particular bio-chemical tests. 

5.0        Frequency:  

            Once in a month.

6.0        Documentation:

            Annexture-1    Culture Purity Record                                     XXX/FRM/000-00

7.0       History of Revision:

Revision No.	Effective Date	Revision details	Reason for revision

Annexure - 1

Culture Purity Record

Name of Culture:

ATCC / MTCC No:

Date of Growth observed:

(on slant)

Date of Validation:

Growth Characteristics on Selective media & Bio-chemical tests;

1.

2.

3.

4.

5.

6.

7.

8.

9.

10.

Remark: Purity of the above culture was confirmed.

Sign & date:

SOP NO.: XXX/SOP/000-00                                                              Format No. XXX/FRM/000-00