SOP for Operation of UV Cabinet.

1.0    Objective:

        To provide written procedure for the operation of UV Cabinet.

2.0          Scope:

          This SOP covers operation of UV cabinet in Quality Control department.

3.0          Responsibility:

        Jr. Research Officer, Research Officer: Responsible for operation, calibration and maintenance of the instrument as per procedure.

               Head of Department: Responsible for calibration and maintenance, timely as per schedule.

               QA Officer/QA Manager: Review the records and governing the document.

4.0          Procedure:

4.1          Ensure that the instrument is clean and free from dust,

4.2          Switch ‘ON’ the mains.

4.3          Switch ‘ON’ the instrument.

4.4       Open the cabinet and place the TLC plate inside it and close the cabinet.

4.5          Three switches are provided on the top right corner of the instrument.

               Switch 1 - For Visible Light.

               Switch 2 - For UV Light at long wavelength (366 nm).

               Switch 3 - For UV Light at short wavelength (254 nm).

4.6          Select the required Light and switch it ‘ON’.

4.7    Observe the plate for visualization of spot from the observation - compartment  (Window).

4.8          Switch ‘OFF’ the instrument when not required.

5.0          Calibration:

5.1        Prepared 0.04 % w/v solution of Sodium Salicylate in alcohol and apply 5 µl spot on a plate coated with silica gel G for lamps emitting emission band with maximum intensity at about 254 nm.

5.2         Examine the spot in a position normal to the radiation. The lamp should be capable of revealing a standard spot of a diameter of about 5 mm on a plate.

5.3           Prepared 0.2 % w/v solution of Sodium Salicylate in alcohol and apply 5 µl spot on a plate coated with silica gel G for lamps emitting emission band with maximum intensity at about 366 nm.

5.4        Examine the spot in a position normal to the radiation. The lamp should be capable of revealing a spot of a diameter of about 5 mm on the plate.

5.5      Enter the details of calibration as per given in Annexure – 1.

6.0          Precautions:

6.1          Do not start at UV rays for a long time.

6.2         Do not use organic solvents for cleaning of the instrument.

6.3       Do not keep the used TLC plate inside the cabinet after completion of analysis.

7.0          Routine maintenance:

7.1          Ensure that the power supply to the instrument is switched OFF before cleaning.

7.2          Clean the instrument with a clean, dry cloth every day.

7.3          Occasionally wet cloth dipped in diluted soap solution can be used. Precaution has to be taken to clean the instrument immediately with dry cloth to remove the moisture.

8.0                    Documentation:

Annexure – 1         UV Cabinet Calibration Record.         XXX/FRM/000

Annexure – 2          UV Cabinet usage log book               XXX/FRM/000

9.0             History of Revision:

Revision No.	Effective Date	Revision details	Reason for revision

Annexure – 1

Calibration of UV Cabinet Calibrated Date   :                                                         Next due date for calibration : Make               :                                                             Model No. :

Calibration : Sodium Salicylate Make  :                                                            Batch No.  :           Spot Observe on Silica Plate Remarks 254 nm (Put ‘Ö’ for positive and ‘X’ for negative result) 366 nm (Put ‘Ö’ for positive and ‘X’ for negative result)

Acceptance Criteria: The lamp should be capable of revealing without doubt a standard spot of Sodium salicylate with a diameter of about 5 mm of chromatographic plate coated with silica gel G. Remarks : Satisfactory / Not Satisfactory

Calibrated by  :                                                                      Checked by      :

SOP No. : XXX/SOP/000-00                   Format No. : XXX/FRM/000-00

                                                                   Annexure – 2

Date	Name of Sample	A. R. No.	Batch No.	Used by	Remark
SOP No. : XXX/SOP/000-00        Format No. : XXX/FRM/000-00