SOP for Operation and calibration of Gas chromatography [FID]

1.0   Objective:

             The objective of this Standard Operating Procedure is to lay down the procedure for Operation and calibration of Gas chromatography.

2.0        Scope:

             This SOP covers procedure for operation and calibration of Gas chromatography

          

3.0        Responsibility:

            Jr. Research Officer, Research Officer: Responsible for operation and maintenance of the instrument as per procedure.

Head of Department: Responsible for maintenance, timely as per   schedule.

QA Officer/QA Manager: Review the records and governing the document.

4.0      Procedure :

4.1     

   I.     Ensure that the instrument is visibly clean and free from dust.

  II. The room temperature should be maintained between 10 to 30°C and relative humidity Should be maintained between 30 to 80 %

III.    Avoid direct exposure to air-blow from air conditioners.

IV. Avoid exposure to direct sunlight and vibrations.

V. Avoid from equipment which generates strong magnetic field.

4.2 Install properly suitable capillary column in GC oven injector & selected detector.

4.3  Open the valve of gas cylinder of Nitrogen, Hydrogen and z-Air. And checked the pressure & leakage.

4.4  Open the Gas pressure valve from the distribution panel.

4.5        Check the backpressure of column.

4.6  Switch on the mains power, stabilizer, GC power & computer power.

4.7 Double click on the instrument1 (online) & instrument1 (offline) icon from the desktop; create new method from instrument1 (offline) and load method in instrument1 (online) from file menu. Apply the chromatographic condition and enter ok. And view the run signal window, After completion of the run, and open the instrument offline select the file and integrate the peak using integration menu, go to calibration task icon and enter the component name and click on ok, then print the chromatogram by clicking print option from file menu.

4.8 Condition the column at 280°C for 10 min. Enter the programming condition of the sample through micro syringe, when G.C shows ready signal.

4.9 Cool the G.C after completion of the analysis & switch it off. Shut down the computer, mains power supply. Close the gas supply from the distribution panel & from cylinder.

5.0       Routine Maintenance:

5.1       Clean the all out side part of instrument.

5.2       Clean the injector and injector liner. Change the glass wool of injector liner. Change the Septa of injector port if  required.

5.3         Check the all connection of gases for leakage.

6.0        Documentation:

             Annexure-1    Gas Chromatography system usage logbook              XXX/FRM/000

             Annexure-2    Gas Chromatography Routine maintenance record    XX/QF/000

7.0       History of Revision:

Revision No.	Effective Date	Revision details	Reason for revision

 Annexure-1

GC USAGE LOGBOOK
Date	Name of sample	Booking No. (A.R. No.)	Batch No.	Analyzed By	Remarks
SOP: XXX/SOP/000-00           Format No. : XXX/FRM/000-00