1.0 Objective:
The objective of this standard
operating procedure is to lay down the procedure for
operating the pH/ISE Meter (Bench).
2.0 Scope:
This SOP covers
operating procedure of pH/ISE METER.
3.0 Responsibility
Jr. Research Officer, Research Officer:
Responsible for operation, calibration and
maintenance of the
instrument as per procedure.
Head of
Department: Responsible for calibration and maintenance, timely as per
schedule.
QA officer/QA Manager:
Review the records and governing the document.
4.0 Procedure:
4.1 Procedure for pH
measurement:
4.1.1 Ensure that instrument
is visibly clean and dust free.
4.1.2 Switch on the pH
meter.
4.1.3 Wait until the instrument finishes the initialization process.
During this process the logo is displayed.
4.1.4
To take a pH measurement
removes the electrode protective cap and simply submerses the electrode and the
temperature probe 3cm into the sample to be tested.
4.1.5
If necessary, press RANGE until
the display changes to the pH
mode.
4.1.6
Enter SETUP menu to select the
pH resolution.
4.2 Procedure for
calibration of pH meter: (Three point
calibration).
4.2.1 Switch on the pH meter.
4.2.2 Press ‘CALIBRATE’
Key to start calibration.
4.2.3 Calibrate the pH
standard solution at 25°C.
4.2.4 Pour small quantities
of selected buffer solution into clean beaker.
4.2.5 For accurate calibration use two beakers for each buffer
solution, the first one for rinsing the electrode and the second one for
calibration.
4.2.6
Remove the protective cap, open
the fill hole and rinse the electrode with some of the buffer solution to be
used for the first calibration point.
4.2.7
Immerse the electrode and the
temperature probe into a buffer solution of your choice (pH 1.68,
4.01, 6.86.7.01, 9.18, 10.01, 12.45) and stir the buffer gently.
4.2.8
The temperature probe should be
close to the pH electrode.
4.2.9
Press CAL
4.2.10 When the reading is
stable and close to the selected buffer (i.e pH 7), confirm function
key is displayed. Press confirms to confirm first point.
4.2.11 The calibrated value and the second expected
value is then displayed on the LCD.
4.2.12 Rinse the electrode
with distilled water and wipe with soft tissue paper and immersed the pH
electrode and the temperature probe into the second buffer solution and stir
gently.
4.2.13 The“6” tag will
blink on the LCD until the reading is stable.
4.2.14 When the reading is stable and close to the selected buffer (i.e pH
4) confirm function key is displayed. Press confirms to confirm
calibration.
4.2.15 The calibrated value
and the third expected value is then displayed on the LCD.
4.2.16 After the second calibration point is confirmed, immerse the pH
electrode and the temperature probe into third buffer solution and stir
gently.
4.2.17 The “6” tag will
blink on the LCD until the reading is stable.
4.2.18 When the reading is stable and close to the selected buffer (i.e pH
9.20) confirm function key is displayed. Press confirms to confirm
calibration.
4.2.19 Then finally rinse the
electrode, finally dip it in sample and record the value.
4.3 Procedure for Ion
Selective Electrode Meter.
4.3.1 Connect an ISE
electrode to the instrument and turn on.
4.3.2 The instrument input
must be channel 2.
4.3.3 Submerse the ISE electrode tip 3cm into the sample to be tested
and wait for the reading to stabilize.
4.3.4
The ISE reading will be
displayed along with the current temperature reading.
4.3.5
The ATC or MTC message is
turned off because ppm reading is not temperature compensated.
4.4 Procedure For calibration
of Ion Selective Electrode Meter.
4.4.1 Connect an ISE
electrode to the instrument and turn on.
4.4.2 Pour small quantities
of selected buffer solution into clean beaker.
4.4.3 For accurate calibration use two beakers for each buffer
solution, the first one for rinsing the electrode and the second one for
calibration.
4.4.4
The HI 3222 permits a 5 point
calibration.
4.4.5
For fluoride electrode the 2PPM
standard is available.
4.4.6
Remove the protective cap from
the ISE electrode.
4.4.7 Immerse the ISE electrode approximately 2cm into the less
concentrated standard solution and stir gently.
4.4.8
Press CAL
4.4.9
The “6” tag
will blink on the LCD until the reading is stable.
4.4.10 When the reading is stable and close to the selected standard
(10PPM), the confirm function key is displayed. Press confirms to confirm
calibration.
4.4.11 The calibrated value
and the second expected value is then displayed on the LCD.
4..4.12 Rinse the electrode with distilled water and
wipe with soft tissue paper and immersed the ISE electrode and the temperature
probe into the second calibration solution and stir gently.
4.4.13 The “6” tag will
blink on the LCD until the reading is stable.
4.4.14 When the reading is stable and close to the selected standard
(1PPM), confirm function key is displayed. Press confirms to confirm
calibration.
4.4.15 The calibrated value and the third expected value is then displayed
on the LCD. After the second calibration point is confirmed, immerse the ISE
electrode and the temperature probe into the third calibration solution and
stir gently.
4.4.16 The“6” tag will
blink on the LCD until the reading is stable.
4.4.17 When the reading is stable and close to the selected standard
(100PPM), the confirm function key is displayed. Press confirms to confirm
calibration.
4.4.18 Then finally rinse the
electrode, finally dip it in sample and record the value.
5.0 Routine maintenance:
5.1 Clean instrument with
dry cloth.
5.2 Clean the electrode
and temperature knob with wet
6.0 Documentation:
5.1 Annexure – 1 - Usage log book XXX/FRM/000
5.2 Annexure
– 2 - Calibration record. XXX/FRM/000
7.0 History of Revision:
Revision No.
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Effective Date
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Revision details
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Reason for revision
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Annexure - 1
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Date
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Booking No.
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Name of sample
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Batch No.
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Used by
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Remark
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SOP No. : XXX/SOP/000 Format No. : XXX/FRM/000-00
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ANNEXURE - 2
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DAILY CALIBRATION DATA
Instrument : pH meter ID
# :
Make : Model :
Month : Frequency : Daily
SOP No. : XXX/SOP/000 Format No. : XXX/FRM/000-00
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