1.0 Objective:
To provide written procedure for
operation and calibration of Colony Counter.
2.0 Scope:
This SOP covers the operation and
calibration of Colony Counter.
3.0 Responsibility:
Microbiologist:
Responsible for operation, calibration and maintenance of the instrument.
Maintain required record.
QA Officer/QAM: Review the
records and governing the document.
4.0 Procedure:
4.1 Ensure that the instrument is visibly clean and free from dust.
4.2
Switch ON the mains, Turn ON the instrument by pressing the reset On/Off
Switch, the display will indicate some numbers.
4.3 Reset
the counter by pressing the reset switch. The counter should now read 0000.
4.4 Place
the Petri plate on the graduated Woulffhneugal glass plate provided on the top
of the instrument.
4.5
Position the magnifying lens to focus the Petri plate or clear
visibility. Ensure that the sufficient margin is left between the magnifying
lens and the Petri plate in order to use the marker conveniently while colony
counting.
4.6 Remove the cap of the Auto-mark and
touch the marking point of the probe on the Petri Dish where a Bacteriological
Colony is located. Press the probe gently. The counter will register a count,
there will be a beep and an ink dot will be marked on the Petri Dish. Continue
till all the colonies are thus counted.
4.7 As a colony is marked with ink while it
is being counted, missing a count or double counting is avoided. When the
counting is completed note the reading on the counter.
4.8 The COUNT push button switch
(next to the RESET switch) may be used to count sparsely populated plates or for
some other counting purpose also.
4.9 If
the Petri plate is not to be contaminated with the marking, then counting can
be done in either of the following
methods :
4.10 If the Petri plate is finely plated (thin
layer) then it can be kept upside down, and marking can be done on the bottom
side (which is on Top)
4.11 The lid can be kept over the plate and
marking may be done on the glass cover. But in this case, as there is a gap
between the media and the marking, the counting should be done in one sitting
without altering the view angle or moving / turning the plate or its cover.
5.0 Refilling the ink
cartridge in the Auto-mark:
5.1 Unscrew the body of the Auto-mark and
remove the cartridge from inside.
5.2 Gently pull, open the cap at the back
of the cartridge.
5.3 Put about five drops of ink and
reverse procedure for refitting.
5.4 The body of the Auto-mark should be
tightened in such a manner, that the gentle push on the probe would register a
count.
6.0 Calibration:
6.1 Operate the instrument as per above
procedure.
6.2 Electronic digit counts the number
accurately, to be tested by using the COUNT switch by manual counting.
6.3 Record the result in the calibration
format as per given in Annexure - 1.
7.0
Routine maintenance:
7.1
Clean instrument with dry cloth.
7.2
Clean magnifying glass with tissue paper.
8.0 Documentation:
8.1 Annexure – 1 Colony Counter Calibration Record
Format XXX/FRM/ 000-00
Annexure
– 2 Colony counter usage log book
XXX/FRM/000
9.0 History of Revision:
Revision No.
|
Effective
Date
|
Revision
details
|
Reason for
revision
|
Annexure
- 1
|
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Calibration Record of
Colony Counter
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Calibration Date
|
:
|
Next Due Date for calibration :
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Instrument ID No.
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:
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Model No. :
|
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Make
|
:
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Sr.
No.
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Counted
Figure
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Display Figure
|
Remark
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1
2
3
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Calibrated by: Checked by:
Date: Date:
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SOP No. : XXX/SOP/000-00 Format No. : XXX/FRM/000-00
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Annexure – 2
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Date
|
Name of Sample
|
A. R. No.
|
Batch No.
|
Used by
|
Remark
|
SOP No. : XXX/SOP/000-00 Format No. :XXX/FRM/000-00
|
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